Conducted in the Puget Sound region of Washington, this cross-sectional study evaluated the sensitivity of self-collected tongue, nasal, and mid-turbinate swab specimens by RT-PCR using samples collected from 530 patients presenting with symptoms of upper respiratory infection. Using nasopharyngeal (NP) swab specimens collected by health care workers for a comparison, the estimated sensitivities of the three types of samples from the patients were 89.8%. This study demonstrates the usefulness of collecting selfcollected tongue, nasal, or mid-turbinate specimens for testing and diagnosis of COVID-19, particularly given the potential for patients to sneeze, cough, or gag during NP swab collection, which puts health care workers at increased risk of transmission.